Summary: Do our genes determine our behavior? Do education and environment have any influence at all? Do humans occupy a unique position in evolution? To clarify these provoking questions, the author takes the reader on an ambitious and entertaining journey through a variety of scientific disciplines. In doing so, he creates an image of human evolution that says that our entire individual knowledge is determined - to the smallest detail - by phylogeny. " ... before shocked humanists discard such radical theses as mere nonsense, they should not completely close their minds to the explanations of a biologist who says that we still know very little about the genetic determination of human behavior and that the invariance of many forms of behavior present in all cultures nourish the suspicion that the determining role of genes is probably far more comprehensive than we have ever dreamed."
Summary: To date, molecular biology techniques have been introduced to all fields of modern biomedical research and are now finding their way into diagnosis and even therapy. This laboratory manual is designed for researchers who have started or are planning to use molecular biology techniques and need reliable protocols for their laboratory work. It is also an ideal tool for teaching basic courses in molecular medicine. The well-described theoretical background of molecular biology also allows the understanding of the principles underlying these techniques. A wide range of topics, including purification, isolation, labelling, hybridization, sequencing and enzymatic modification of DNA or RNA, polymerase chain reaction, cloning, libraries, and protein techniques, are described for the immediate application in the laboratory.
Author(s): Hummel, S ISBN: 3-540-43037-7 Publisher:
Price: $67.08
Summary: Ancient DNA Typing is a comprehensive manual about the analysis of ancient and degraded DNA. It is an equally useful textbook and working tool for various disciplines such as: Anthropology, Archaeology, Forensic Medicine, Conservation Biology, Environmental Sciences and Food Engineering.
The reader benefits from a clear division of strategies, methods and applications with many interesting examples of the analysis of historic skeletal and cultural remains. The book contains a detailed protocol chapter which serves as a lab manual with the most important methods for DNA extraction, PCR and electrophoretic analysis of degraded samples. The appendix helps to set up a PCR lab specializing in ancient DNA research and answers the most frequent questions concerning technical problems in investigations of degraded DNA.
Summary: Recent advances in large-scale DNA sequencing technology have made it possible to sequence the entire genome of an organism. Attention is now turning to the analysis of the product of the genome, the proteome, which is the set of proteins being expressed by a cell. Two-dimensional gel electrophoresis can be used to create cellular protein maps which give a quantitative and qualitative picture of the proteome. Mass spectrometry is the method of choice for the rapid large-scale idenfification of these proteomes and their modifications. An understanding of these methods is critical for scientists in the "Post-Genome" era.
Summary: This complete and practical manual on expression measurement using DNA arrays covers the existing methods (from nylon macroarrays to oligonucleotide chips) and includes detailed protocols. It has been written by practising scientists who have experienced the difficulties involved in actually using microarrays, and provides helpful advice and hints on setting up these powerful but sometimes tricky methods. Software, data mining procedures and probable future developments, which should be useful to any practising scientist interested in expression measurement, are also covered in this book. It also provides detailed protocols as well as many helpful hints to achieve experimental success and to avoid pitfalls.
